C9995
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 | Immunoblotting Cell lysates of HEK-293T overexpressing human cullin-4B (Lanes 1-3) and of HEK-293T non transfected (Lane 4), were separated on SDS-PAGE, blotted with Anti-Cullin-4B (Cat. No. C9995) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 1.5 μg/mL 2. Antibody: 0.75 μg/mL 3. Antibody: 1.5 μg/mL + cullin-4B immunizing peptide (human, 40-59) 4. Antibody: 1.5 μg/mL |
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C9995
Sigma
Anti-Cullin-4B antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Cul4B, Anti-MRXHF2, Anti-MRXSC, Anti-SFM2 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 40-59 of human cullin-4B, conjugated to KLH. The corresponding sequence is identical in rat and mouse cullin-4B and is not found in the homolog cullin-4A. |
| Physical form | Solution in 0.01 M phosÂphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~100 kDa |
| species reactivity | human |
| concentration | ~1.0 mg/mL |
| application(s) | immunoblotting: 0.7-1.5 μg/mL using HEK-293T cells expressing human Cullin-4B. |
| Gene Information | human ... CUL4B(8450) |
| rat ... 302502(Cul4b) | |
| mouse ... Cul4b(72584) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 1 |
Related Products
| Related product | C0371, Anti-Cullin-4A antibody produced in rabbit |
